Acceptor reactions and mechanism of Leuconostoc mesenteroides B-512FM dextransucrase
نویسنده
چکیده
14 INTRODUCTION 16 MATERIALS AND METHODS 19 Carbohydrates and reagents 19 Enzyme 19 Enzyme digest conditions 20 Digest conditions to obtain Hanes-Woolf plots 20 Digest conditions to obtain Michaelis plots 20 Determination of the amount of dextran synthesized 21 RESULTS AND DISCUSSION 22 REFERENCES 3 0 iii PAPER II INTERPRETATION OF DEXTRANSUCRASE INHIBITION AT HIGH SUCROSE CONCENTRATIONS 3 9 ABSTRACT 4 0 INTRODUCTION 41 EXPERIMENTAL 44 Chromatography 44 Enzyme 4 4 Enzyme digest conditions 4 5 RESULTS 4 7 DISCUSSION 49 REFERENCES 54
منابع مشابه
Induction and transcription studies of the dextransucrase gene in Leuconostoc mesenteroides NRRL B-512F.
Dextransucrase production by Leuconostoc mesenteroides NRRL B-512F in media containing carbon sources other than sucrose is reported for the first time. Dextransucrases were analyzed by gel electrophoresis and by an in situ activity assay. Their polymers and acceptor reaction products were also compared by (13)C nuclear magnetic resonance and high-performance liquid chromatography techniques, r...
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